Figure 2.

Bin1^mck−/− mice develop most of the histopathological hallmarks of CNM in TA

(A) Representative H&E and SDH images of TA from Bin1^fl/fl and Bin1^mck−/− mice at 8 weeks of age. Bin1^mck−/− muscle sections present SDH accumulations in the center of fibers (arrows; n = 3). Scale bar, 50 μm. (B) MinFeret perimeter distribution of TA fibers (n = 4–5). (C) The percentage of large fibers with MinFeret diameter higher than 40 μm is reduced in Bin1^mck−/− compared to Bin1^fl/fl controls (n = 4–5; Student’s t test). (D) Myofiber circularity distribution in TA; a value of 0 corresponds to a line and a value of 1 to a circle (n = 4–5). Bin1^mck−/− fibers are rounder. (E) Percentage of fibers with internalized nuclei reveals no difference between Bin1^mck−/− and Bin1^fl/fl muscles (n = 4–5; Student’s t test). Graphs represent mean ± SEM. ∗∗p < 0.01.