Figure 6.

Dnm2 downregulation rescues the TA CNM histopathological hallmarks of Bin1^mck−/− mice

(A and B) Representative H&E and SDH images of TA from PBS and ASO-treated Bin1^fl/fl and Bin1^mck−/− mice at 8 weeks of age (n = 4–6). Scale bar, 50 μm. ASO-treated Bin1^mck−/− mice do not present the central SDH accumulations seen in PBS-treated Bin1^mck−/− mice (arrows). (C) MinFeret perimeter distribution of TA fibers from PBS- and ASO-treated Bin1^fl/fl and Bin1^mck−/− mice (n = 4–5). (D) Myofiber circularity from ASO-treated Bin1^fl/fl− and Bin1^mck−/− TA; a value of 0 correspond to a line and a value of 1 to a circle (n = 4–5). (E) Percentage of fibers with MinFeret diameter higher than 40 μm are comparable in PBS- and ASO-treated Bin1^fl/fl and Bin1^mck−/− mice (n = 4–7; one-way ANOVA with Tukey’s post hoc test). Graphs represent mean ± SEM. ∗∗p < 0.01; ∗p < 0.05.