Figure 3.

ID was associated with increased SPNS2 expression

(A and B) N and T tissues in the liver were sectioned from the orthotopically administrated mice in Figure 2B. n = 3. (A) mRNA and (B) protein expression of SPNS2 and iron-related genes were determined. (C and D) Liver and lung tissues were sectioned from the orthotopically administrated (C) C57BL/6 mice in Figure 2B or (D) the WT (TFRC^+/+) and TFRC-knockout (TFRC^–/+) mice in in Figure 2E. The percentage of lymphocyte subsets and that of effective T cells in the liver (left) and lung (right) were determined by flow cytometry assay. Effective: CD 44^high, CD62^low. n = 3. (E) The mRNA and (F) protein expression of TFRC and SPNS2 in the human M and NM HCC tumors in Figure 1D. N = 15. Data were presented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01 versus IA diet, versus N mice, or versus NM. SPNS2, transporter spinster homolog 2.