Figure 6.
IFNα induces CD169 upregulation on macrophages and inhibits tumor progression in vivo
(A–G) WT C57BL/6 mice were injected subcutaneously with 1 × 106 Hepa1-6 cells. Then, 10 μg of IFNα-IgG or PBS (no treatment control [NTC]) was administered intravenously on day 8, and tumor tissues were collected on day 11; n = 10. (A–E) Immunocytes were isolated from freshly dissociated tumor specimens, and they were analyzed by multiparametric flow cytometry analysis. Represented FACS data were analyzed using the tSNE algorithm (A), and statistical analysis was conducted as shown in (B)–(E). (F) Tumor volume. (G) Tumor weight. Data represent mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001; NS, not significant; multiple t test (B–E) and unpaired Student’s t test (F and G).