Figure 1.

Hepatocyte-specific FBP1 loss restrains NK cells in DEN-induced liver tumorigenesis

(A) Flow chart of generating DEN-induced liver cancer mouse model with or without hepatic Fbp1 deletion. (B and C) The mRNA (B) and protein (C) expression levels of FBP1 in indicated mouse livers. (D−H) Representative liver images (scale bar, 1 cm) (D), the number of liver surface tumor nodules (E), representative H&E staining (scale bar, 100 μm) (F), numbers of microscopic tumors (G), and Ki67⁺ cells per field (H) of liver tissues from DEN-treated GFP and Cre mice at 24 weeks; n = 5. (I) Flow cytometry analysis of activated NK cells (CD3⁻NKP46⁺), B cells (B1a cells: CD19⁺ IgM⁺CD43⁺CD5⁺, B1b cells: CD19⁺ IgM⁺CD43⁺CD5⁻, B2 cells: CD19^+t IgM^+tCD43⁻CD5⁻, Regulatory B (Breg) cells: CD19^+tCD5^+tCD1d^+t), T cells (CD4 cells: CD3^+tCD4^+t, CD8 cells: CD3^+tCD8^+t, γδT cell receptor (TCR) cells: CD3^+tγδTCR^+t), and macrophages (CD11b cells: F4/80^+tCD11b^+t, CD206 cells: F4/80^+tCD206^+t) in aforementioned liver tissues, n = 3. (J) Representative NKp46 immunohistochemical staining images (let) and quantifications (right) in 24-week DEN/GFP and DEN/Cre livers. n = 3. Scale bar, 100 μm. Data are presented as mean ± SD. Unpaired Student’s t test, ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001. See also Figures S1 and S2.