Figure 2.

CDP effectively inhibits hTLR9 and hTLR3 activation by nucleic acids in a cell culture reporter assay

(A) HEK-Blue hTLR9 cells were incubated with or without the TLR9 agonist CpG ODN2006 (1μM) and increasing concentrations of CDP. CDP effectively inhibits TLR9 activation with an IC₅₀ value of 26.6 μg/mL, returning to baseline when added at 50 μg/mL and above. (B) HEK-Blue hTLR3 cells were incubated with or without the TLR3 agonist high-molecular-weight (HMW) poly(I:C) (1 μg/mL) and increasing concentrations of CDP. CDP effectively inhibits TLR3 activation with an IC₅₀ value of 11.6 μg/mL, returning to baseline at 20 μg/mL and above. (C) HEK-Blue hTLR4 cells were incubated with or without the TLR4 agonist lipopolysaccharide (LPS; 100 ng/mL) and increasing concentrations of CDP. CDP did not affect LPS activation of hTLR4. Whiskers represent max and min of replicates.