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. 2021 Oct 19;30(2):763–781. doi: 10.1016/j.ymthe.2021.10.012

Figure 2.

Figure 2

Exosomes accumulated in activated pericytes and obstructed kidney both in vitro and in vivo

(A) (Left) Representative electron micrograph of exosomes. Scale bar, 100 μm. (Middle) Nanoparticle tracking analysis. (Right) CD63 and CD81 levels were assessed using western blotting. Scale bar, 50 μm (n = 3). (B) The exosome content of MSC-CM was detected by luciferase reporter. Data are the mean ± SD (n = 3). (C) Representative images of PKH67 (green) and PDGFRβ (red) staining in vitro. The right panel shows the quantification. Scale bar, 75 μm. Control, pericytes without TGFβ1 stimulation. 0 h, 24 h, 48 h, pericytes without TGFβ1 stimulation for 0 h, 24 h, 48 h. (D) Ex vivo UUO mouse imaging at 0 h, 6 h, 12 h, 24 h, 72 h, 168 h, and 240 h.