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. 2021 Oct 19;30(2):763–781. doi: 10.1016/j.ymthe.2021.10.012

Figure 3.

Figure 3

MSCs inhibited pericyte activation and RIF mainly through secreting exosomes

(A) FUT8 levels were assessed using western blotting. The bottom panel shows the quantification. Scale bar, 50 μm. Data are the mean ± SD (n = 3). Control, pericytes without TGFβ1 stimulation. TGFβ1+MSCs, 48-h TGFβ1-stimulated pericytes plus MSCs; TGFβ1+Exo, 48-h TGFβ1-stimulated pericytes plus exosomes; TGFβ1+CM, 48-h TGFβ1-stimulated pericytes plus CM; TGFβ1+CM(−)Exo, 48-h TGFβ1-stimulated pericytes plus CM(−)Exo. (B) FUT8 activity examined by HPLC. Data are the mean ± SD (n = 3); S indicates the peptide substrate; P is the fucosylation product. (C) Representative images of PDGFRβ (green) and LCA (red) staining, and α-SMA (green) and LCA (red) staining. The right panel shows the quantification. Scale bar, 75 μm. Data are the mean ± SD (n = 3). (D) Representative morphological alterations in pericytes. Scale bar, 200 μm. (E) Representative images of PDGFRβ (green) and α-SMA (red) staining. The bottom panel shows the quantification. Scale bar, 75 μm. α-SMA levels were assessed using western blotting. The right panel shows the quantification. Scale bar, 50 μm. Data are the mean ± SD (n = 6). (F) Representative images of PDGFRβ (green) and LCA (red) staining, and α-SMA (green) and LCA (red) staining. The bottom right panel shows the quantification. Scale bar, 75 μm. Data are the mean ± SD (n = 6). Control, C57 mice without UUO surgery. UUO+MSCs, 7 days after MSC injection into UUO mouse tail vein; UUO+Exo, 7 days after Exo injection into UUO mouse tail vein; UUO+CM, 7 days after CM injection into UUO mouse tail vein; UUO+CM(−)Exo, 7 days after CM(−)Exo injection into UUO mouse tail vein. (G) Representative images of PDGFRβ (green) and CD31 (red) staining, and PDGFRβ (green) and α-SMA (red) staining. The bottom panel shows the quantification. Scale bar, 75 μm. Data are the mean ± SD (n = 6). (H) Representative images of Masson's trichrome and PAS staining of UUO mouse kidney sections. The bottom panel shows the quantification. Scale bar, 200 μm. Data are the mean ± SD (n = 6).