Figure 3.

PD-L1 detection was significantly enhanced after deglycosylation. (A) H-score-M of PD-L1, (B) TPS of PD-L1, (C) H-score of PD-L1-M+C, and (D) IC of PD-L1 with or without deglycosylation in all samples. (E) H-score of PD-L1-M, (F) TPS of PD-L1, (G) H-score-M+C of PD-L1, and (H) IC of PD-L1 with or without deglycosylation in subgroup according to the best response for atezolizumab. Fold changes (FC) is the subtraction of the mean score between deglycosylated and natural value of each measurements. (I) Quantification of TPS (upper) and H-score-M (lower) of IHC staining for nine TNBC patients processed with or without PNGase F pretreatment. P-value was estimated using Wilcoxon signed rank test.