Figure 3. Mitochondrial alterations and ROS production. SIM-A9 and 4T1 cells were left without treatment (control) or treated with CC50 of EPI and CTX for 24 h and assessed by flow cytometry. A) Representative histogram of mitochondrial membrane potential measured by DIOC6 staining (left), bars represent the mean ± SD (right). B) Representative histogram of ROS production measured with DCFDA (left), bars represent the mean ± SD (right). C) Representative histogram of Annexin V staining, in cells pre-treated or not with the antioxidant NAC and then treated with EPI or CTX (left), bars represent the mean ± SD (right).
