Fig. 2.

Differing amounts of exposure to compression reveal distinct metabolic profiles. Comparison of samples exposed to compression reveal metabolomic profiles of chondrocytes between 15 and 30 min of compression differ. (A) Samples compressed at different times separate into distinct clusters by HCA as illustrated in the dendrogram. (B) PCA displays some clustering of samples within their respective cohorts: chondrocytes exposed to compressive forces for 15 min (orange) and 30 min (yellow). Control chondrocytes that were not exposed to mechanical stimuli are displayed for comparison purposes (pink). PCA is shown as a scatterplot of the first two PCs (PC1 and PC2), which account for 23.2% and 15.7% of the overall variation in the dataset, respectively. (C) PLS-DA finds clear separation between samples. PLS-DA is visualized as a scatterplot of the top two components, which account for 16.8% and 13.6% of the overall variation in the dataset. n=5 samples per group.