Fig. 3.

Differing amounts of shear force reveal distinct metabolic profiles. Metabolomic profiles of chondrocytes exposed to 15 and 30 min of shear force differ. (A) Samples compressed at different times separate into weakly distinct clusters by HCA as illustrated in the dendrogram. (B) PCA displays some clustering of samples within their respective cohorts: chondrocytes exposed to shear force for 15 min (green) and 30 min (blue). Control chondrocytes that were not exposed to mechanical stimuli is displayed for comparison purposes (pink). PCA is shown as a scatterplot of the first two PCs (PC1 and PC2), which account for 19.3% and 14.8% of the overall variation in the dataset, respectively. (C) PLS-DA finds clear separation between samples. PLS-DA is visualized as a scatterplot of the top two components, which account for 10.5% and 13.7% of the overall variation in the dataset. n=5 samples per group.