Figure 11.

Histological analysis of longitudinal optic nerve sections and significant correlations at 37 dpi. (a) Quantification of microglia/macrophage cells (mm²) in optic nerves stained with Iba 1. (b) Correlation between VEP latency change (%) and microglia/macrophage cells. (c) Quantification of axonal loss (%) in optic nerves stained with SMI 312. (d) Correlation between VEP latency change and axonal loss. (e) Quantification of demyelination (%) in optic nerve stained with LFB. (f) Correlation between VEP latency change and demyelination. (a, c, e) Healthy (black columns, n = 13 eyes); EAE EON (light grey columns, n = 14 eyes); EAE LON (dark grey bars, n = 14 eyes). Error bars represent the SE. Hashes represent significant differences between EAE EON and Healthy; triangles represent significant differences between EAE LON and Healthy. (a, c, e) Pairwise comparisons of predicted marginal means were performed according to the concept of least‑squares means (*p < 0.01;**p < 0.001;***p < 0.0001). (b, d, f) Spearman’s correlations.