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. 2022 Feb 7;15:13. doi: 10.1186/s13068-022-02111-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Design and evaluation of the transcription and protein regulation system. a Design of the transcription and protein repression system b Evaluation of the integrated TPRS. c Evaluation of the control design of the transcription and protein repression system. d Design of the transcription and protein activation system. e Evaluation of the TPAS. f Evaluation of the (TPAS) control design g Growth profile of the TPRS and TPAS containing strains under dark and light conditions. h, i Evaluation of the TPRS and TPAS component performance: h Assessment of the benefits of TPRS, transcription, and protein repression system and its modular components PRU, protein repression unit; and TRU, transcription repression units. i Assessment of the TPAS, transcription, and protein activation system, together with the components; PAU, protein activation unit; TAU, transcription activation units