Table 1.
Strains used in this study
| Strains/Plasmids | Relevant characteristics | Source |
|---|---|---|
| Strains | ||
| K1 | E. coli MG1655 carrying pJ01-TetR | This study |
| TRU(N26/C27) | E. coli MG1655 carrying pJ01-TetR-26, Ptet-GFP | This study |
| TRU(N36/C37) | E. coli MG1655 carrying pJ01-TetR-36, Ptet-GFP | This study |
| TRU(N46/C47) | E. coli MG1655 carrying pJ01-TetR-46, Ptet-GFP | This study |
| TRU (N68/C69) | E. coli MG1655 carrying pJ01-TetR-68, Ptet-GFP | This study |
| TRU (N99/C100) | E. coli MG1655 carrying pJ01-TetR-99, Ptet-GFP | This study |
| TRU(N104/C105) | E. coli MG1655 carrying pJ01-TetR-104, Ptet-GFP | This study |
| TRU(N124/C125) | E. coli MG1655 carrying pJ01-TetR-124, Ptet-GFP | This study |
| TRU(N166/C167) | E. coli MG1655 carrying pJ01-TetR-166, Ptet-GFP | This study |
| TRU(N167/C168) | E. coli MG1655 carrying pJ01-TetR-167, Ptet-GFP | This study |
| TRU(N169/C170) | E. coli MG1655 carrying pJ01-TetR-169, Ptet-GFP | This study |
| TRU(N179/C180) | E. coli MG1655 carrying pJ01-TetR-179, Ptet-GFP | This study |
| XN | E. coli MG1655, ΔLacI | This study |
| TAU(N124/C125) | XN carrying pJ01-TetR-124, Ptet LacI-GFP | This study |
| TAU(N46/C47) | XN carrying pJ01-TetR-46, Ptet LacI-GFP | This study |
| TAU(N169/C170) | XN carrying pJ01-TetR-169, Ptet LacI-GFP | This study |
| TAU(N179/C180) | XN carrying pJ01-TetR-179, Ptet LacI-GFP | This study |
| TAU(N26/C27) | XN carrying pJ01-TetR-26, Ptet LacI-GFP | This study |
| TAU(N166/C167) | XN carrying pJ01-TetR-166, Ptet LacI-GFP | This study |
| TAU(N167/C168) | XN carrying pJ01-TetR-167, Ptet LacI-GFP | This study |
| TAU(N68/C69) | XN carrying pJ01-TetR-68, Ptet LacI-GFP | This study |
| PRU-118 | E. coli MG1655 carrying TEVp-118, Ptet-(TeF)GFP | This study |
| PRU-118-NW | E. coli MG1655 carrying TEVp-118-NW, Ptet-(TeF)GFP | This study |
| PAU-118 | E. coli MG1655 carrying TEVp-118, Ptet-GFP(LAA) | This study |
| PAU-118-NW | E. coli MG1655 carrying TEVp-118-NW, Ptet-GFP(LAA) | This study |
| TPRS | E. coli MG1655 carrying pJ01-TetR(166)-TEVp(118), Ptet-(TeF)GFP | This study |
| TPAS | XN carrying pJ01-TetR(166)-TEVp(118), Ptet-(TeF)LacI-GFP(DAS) | This study |
| S4 | E. coli MG1655, ΔptsHIcrr::Zmglf, ΔaroL::tktA, ΔaroK, PJ23119-GB | Lab stock |
| S5 | S4, ΔaroK::rpE, ΔyidB | This study |
| S6 | S5 carrying pJ01-TetR(166)-TEVp(118), NX1 | This study |
| S7 | S5 carrying pJ01-TetR(166)-TEVp(118), NX2 | This study |
| S8 | S5 carrying pJ01-TetR(166)-TEVp(118), NX3 | This study |
| Plasmids | ||
| pJ01 | PJ23119 promoter, pMB1 ori, AmpR | Lab stock |
| pJ01-TetR-26 | pJ01-containing B0034RBS, TetR(N26)-VVD, VVD-TetR(C27) | This study |
| pJ01-TetR-36 | pJ01-containing B0034RBS, TetR(N36)-VVD, VVD-TetR(C37) | This study |
| pJ01-TetR-46 | pJ01-containing B0034RBS, TetR(N46)-VVD, VVD-TetR(C47) | This study |
| pJ01-TetR-68 | pJ01-containing B0034RBS, TetR(N68)-VVD, VVD-TetR(C69) | This study |
| pJ01-TetR-99 | pJ01-containing B0034RBS, TetR(N99)-VVD, VVD-TetR(C100) | This study |
| pJ01-TetR-102 | pJ01-containing B0034RBS, TetR(N104)-VVD, VVD-TetRC105) | This study |
| pJ01-TetR-124 | pJ01-containing B0034RBS, TetR(N124)-VVD, VVD-TetR(C125) | This study |
| pJ01-TetR-166 | pJ01-containing B0034RBS, TetR(N166)-VVD, VVD-TetR(C167) | This study |
| pJ01-TetR-167 | pJ01-containing B0034RBS, TetR(N167)-VVD, VVD-TetR(C168) | This study |
| pJ01-TetR-169 | pJ01-containing B0034RBS, TetR(N169)-VVD, VVD-TetR(C170) | This study |
| pJ01-TetR-179 | pJ01-containing B0034RBS, TetR(N179)-VVD, VVD-TetR(C180) | This study |
| pJ01-TetR | pJ01-containing B0034RBS, TetR(Full length) | This study |
| PTet-1 | Ptet, p15A ori, CmR, TetR | Lab stock |
| PTet-2 | Ptet, p15A ori, CmR | This study |
| Ptet-GFP | PTet-2 containing B0034RBS, GFP | This study |
| Ptet LacI-GFP | PTet-2 containing Ptet, B0034RBS, LacI, Ptrc, B0034RBS, GFP | This study |
| TEVp-118 | pJ01 containing Pj23119, B0034RBS, TEVp(N118)-VVD, VVD-TEVp(C180) | This study |
| TEVp-118-NW | pJ01 containing Pj23119, B0034RBS, VVD-TEVp(N118), VVD-TEVp(C180) | This study |
| Ptet-(TeF)GFP | PTet-2 containing Ptet, B0034RBS, (TeF)GFP | This study |
| Ptet-GFP(LAA) | PTet-2 containing Ptet, B0034RBS, GFP(LAA) | This study |
| pJ01-TetR(166)-TEVp(118) | pJ01 containing B0034RBS, TetR(N166)-VVD, VVD-TetR(C167), Pj23119-VVD-TEVp(N118), VVD-TEVp(C180) | This study |
| Ptet-(TeF)LacI-GFP(DAS) | PTet-2 containing Ptet, B0034RBS, (TeF)LacI, GFP fused with DAS tag | This study |
| pJ01-TEVp | pJ01 containing Pj23119, B0034RBS, TEVp(Full length) | This study |
| PJ01-GB | pJ01 containing Pj23119, B0034RBS, AroG, AroB | This study |
| NX-1 | PTet-2 containing Ptet, B0034RBS, (TeF)AroK | This study |
| NX-2 | PTet-2 containing Ptet, B0034RBS, (TeF)LacI, Ptrc, B0034RBS, AroG(DAS), AroB(DAS) | This study |
| NX-3 | PTet-2 containing Ptet, B0034RBS, (TeF)AroK, (TeF)LacI, Ptrc, B0034RBS, AroG(DAS), AroB(DAS) | This study |