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Designed primer sequences with the respective annealing temperature and amplicon size for gene amplification
| Target gene | Primer name | Sequence (5′-3′) | Annealing temperature (°C) | Amplicon size (bp) |
|---|---|---|---|---|
| 12S rRNA | Tre12S-F | GTGCCAGCADYYGCGGTTA | 55 | 371 |
| Tre12S-R | AGCAGCAYATHGACCTG | |||
| 16S rRNA | CesTre16S-F | GTGYDAAGGTAGSATAAT | 56 | 379 |
| CesTre16S-R | CCGGTYTYAACTCARCTCAT |
