Table 2.

Antibodies used for immunohistochemistry assay

Antibody	Source	Clone and manufacturer	Antigen retrieval solution pH	Dilution and incubation	Positive control
Anti-p53	Mouse monoclonal IgG1	DO-7, Dako, Glostrup, Denmark	10 mM Citrate buffer pH 6.0	1:50, overnight at 4 °C	Breast cancer sections with p53-positive nuclear staining
Anti-p16INK4a	Mouse monoclonal IgG1	JC8, Santa Cruz Biotechnology, California, USA	10 mM Tris–EDTA buffer pH 9.0	1:50, overnight at 4 °C	Formalin-fixed HeLa cell blocks
Anti-Ki-67	Mouse monoclonal IgG1	MIB-1, Dako, Glostrup, Denmark	10 mM Citrate buffer pH 6.0	1:100, overnight at 4 °C	Intestine tissue sections
Anti-NF-κB (p65)	Mouse monoclonal IgG1	Santa Cruz Biotechnology, California, USA	10 mM Citrate buffer pH 6.0	1:400, 1 h at room temperature	Formalin-fixed HeLa cell blocks