Fig. 1.

NOD1 and NOD2 are upregulated in human CSCC tissues and associated with poor survival. A Partial heat map showing differentially expressed NLR genes including NOD1 and NOD2 in the CSCC (n = 4) and normal cervix (n = 6) tissues. B NOD1 and NOD2 mRNA copy numbers in unpaired CSCC tissues (NOD1, n = 59; NOD2, n = 24) and normal cervix (NOD1, n = 33; NOD2, n = 31). C NOD1 and NOD2 mRNA expression in the CSCC (NOD1, n = 75; NOD2 n = 75) and normal cervix samples (non-tumoral adjacent tissue, NOD1, n = 188; NOD2 n = 188) extracted from TCGA database. D Representative immunofluorescence images showing co-staining of AE1/AE3 and NOD1/NOD2 in paired CSCC tumors and normal cervix tissues (data were from two independent experiments with eight samples). E Representative IHC images showing in situ expression of NOD1and NOD2 in paired human CSCC tissues of different pathological stages (early and late stages and LVSI) and adjacent non-tumor tissues (scale bar = 100 μm and magnification—× 10 or × 20). F–H IHC scores of NOD1 and NOD2 in F paired tumor and adjacent non-tumor tissues (NOD1, n = 75; NOD2, n = 70), G tumors with and without LVSI (NOD1: LVSI = 58, non-LVSI = 45; NOD2: LVSI = 48, non-LVSI = 49), and H tumors with and without LM (NOD1: LM = 48, non-LM = 48; NOD2: LM = 55, non-LM = 39). I Kaplan-Meier curves showing overall survival of CSCC patients demarcated on the basis of in situ NOD1 expression (http://www.proteinatlas.org). J NOD1 and NOD2 mRNA levels and representative immunofluorescence images showing respective protein levels in Siha and CasKi cell lines. K Immunofluorescence images showing respective NOD1 and NOD2 protein levels in primary CSC cells. For cell lines, the experiments were performed in two wells with three replicates; for primary cells, the experiments were performed by two independent experiments with four samples; the picture is a representative one. *P < 0.05, ** P < 0.01, *** P < 0.001