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. 2022 Feb 7;19:38. doi: 10.1186/s12974-022-02397-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Gas6 does not affect microglial phagocytosis of amyloid beta in vivo or in vitro. A Ten-month-old APP/PS1 and nontransgenic littermates injected with AAV-Gas6 or AAV-control at 9 months of age were intraperitoneally injected with MX-04 24 h prior to sacrifice. Brains were processed and microglia (CD45lo/CD11b+) were analyzed by flow cytometry. Representative plots of gating strategy. B, C MX-04 + microglial populations showing percent of MX-04+ cells and relative median fluorescence per cell. N = 2–4 per group. Error bars represent mean ± SEM. D Primary microglia from Axl^+/− mice were treated with rmGas6 (50 nM) and beta-amyloid (1–42) HiLyte Fluor 488 for 15 or 30 min and analyzed by flow cytometry. N = 4–5 biological replicates per group. Error bars represent mean ± SEM. E Primary microglia from Axl + / − or Axl−/− P0-P2 pups were treated with rmGas6 for 15, 30, 60, or 120 min and analyzed by flow cytometry. N = 4–6 biological replicates per group. Error bars represent mean ± SEM