Fig. 6.
PTPN14 regulates sensitivity to sorafenib through YAP under hypoxia. Representative immunofluorescence staining of the localization of YAP in HepG2 cells with stable PTPN14 knockdown (A) and SK-Hep1 cells with PTPN14 stable overexpression (B) under normoxia (20% O2) or hypoxia (2% O2) for 24 h. Scale bar = 20 μm. C The expression of p-YAP and total YAP in the whole cell lysate (WCL) and nuclear fractions in HepG2 cells with stable PTPN14 knockdown and SK-Hep1 cells with PTPN14 stable overexpression under hypoxic conditions (2% O2) for 24 h was examined by western blotting. D The IC50 was measured using an MTT assay after 72 h of sorafenib treatment. *p < 0.05; **p < 0.01; ***p < 0.001