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. 2022 Feb 7;19:35. doi: 10.1186/s12974-022-02393-2

Fig. 2.

Fig. 2

Extract, culture, and sorting of BMSCs, and identification and label of BMSC-exos. A Bone-marrow primary adherent cells before sorting (P0, Primary cells; P1, passage 1; P2, passage 2; P3, passage 3). B CD44+/CD45 (96.4%) and CD11b (99.2%) BMSCs were sorted out by flow sorting, BMSCs grew into fibrous and colony-like growth, scale bar 100 μm. C Exosome morphology under transmission electron microscope. D Western blot photos of exosome markers. E Fluorescence imaging of exosome labeled with PKH26