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. Author manuscript; available in PMC: 2023 Feb 3.
Published in final edited form as: Cell Stem Cell. 2022 Jan 18;29(2):298–314.e9. doi: 10.1016/j.stem.2021.12.007

Figure 1. In vivo shRNA screening identifies Traf6 in the transformation of pre-leukemic HSPC.

Figure 1.

(A) Outline of in vivo shRNA screen to identify cooperating genes/pathways in the transformation of Tet2−/− c-Kit+ BM cells. (B) Kaplan Meier survival analysis of mice transplanted with Tet2−/− cells transduced with the shRNA library (n = 4 mice per pool), shp53 (n = 3), shNCSTN (n = 3), or shRenilla (n = 4). (C) Flow cytometry analysis of dsRed-positive PB cells isolated from mice transplanted with Tet2−/− cells transduced with the shRNA library. (D) Violin plot of the normalized counts for the shRNAs in the plasmid pools (P1-P4), progenitor cells before transplantation (T0) and leukemic cells (L1-L4) collected from moribund mice. Enrichment of shRNAs targeting Traf6 are indicated. (E) Pie charts of the cells transduced with Pool 2 before transplantation and leukemia collected from 4 mice. The area of each shRNA corresponds to the fraction of total reads.