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. 2022 Feb 7;10(2):e003382. doi: 10.1136/jitc-2021-003382

Figure 3.

Figure 3

HMBD-002 binds with high affinity and specificity to a species-conserved epitope on recombinant and cell surface expressed VISTA orthologs. (A) Binding specificity of HMBD-002 by ELISA using human B7 family antigens as indicated. Data shown are mean n=2 measurements and error bars are SEM. (B) ELISA binding of HMBD-002 to VISTA orthologs; human, NHP, rat or mouse VISTA. Data shown are mean of n=3 measurements and error bars are SEM. (C and D) Binding specificity of HMBD-002 to cell surface expressed VISTA by flow cytometry on overexpressing HEK293T cells or wild type HEK293T (C) and CD11b+ myeloid cells isolated from human, NHP, rat or mouse PBMCs (D) Data shown are mean of n=3 measurements and error bars are SEM. NHP, non-human primate; PD-1, programmed cell death protein-1; PD-L1, programmed death-ligand 1; VISTA, V-domain Ig suppressor of T cell activation; WT, wild type.