FIG 6.

BbATG3 is required for autophagy in B. bassiana under starvation. (A) Representative images of the autophagic process. Conidia of the indicated strain were inoculated into SDB, and the resultant mycelia were stressed under starvation in mineral solution for 3 h. Autophagic bodies (indicated with “A”) in mycelial vacuoles were examined by transmission electronic microscopy. Scale bars represent 0.2 μm. (B) Fluorescent protein indicating the autophagic process. A hybrid gene, ATG8-GFP, was transformed into the wild-type and ΔBbatg3 mutant strains, and the protein products show the trafficking of autophagosomes/autophagic bodies. Ablation of BbATG3 interrupted the translocation of autophagosome from cytoplasm to vacuoles. Scale bars indicate 10 μm.