TABLE 2.
Comparison of MAb clones 5-D8/1 and 2E11 for the rapid Detection of enteroviruses by AG-CCA
| Virus type | Positive result witha:
|
|||||
|---|---|---|---|---|---|---|
| AG-CCA > TCID50
|
AG-CCA < TCID50
|
AG-CCA = TCID50
|
||||
| 5-D8/1 | 2E11 | 5-D8/1 | 2E11 | 5-D8/1 | 2E11 | |
| Poliovirus | ||||||
| 1 | Yes | Yes | ||||
| 3 | Yes | Yes | ||||
| Echovirus | ||||||
| 6 | Yes | Yes | ||||
| 9 | Yes | Yes | ||||
| 11 | Yes | Yes | ||||
| 30 | Yes | Yes | ||||
| Coxsackievirus | ||||||
| A9 | Yes | Yes | ||||
| A16 | Yes | Yes | ||||
| B3 | Yes | Yes | ||||
| B5 | Yes | Yes | ||||
a
The AG-CCA and conventional RhMk TC assay systems were inoculated with enterovirus stock cultures at viral end point concentrations. TCID50, 50% tissue culture infective dose. AG-CCA > TCID50, detection of enterovirus antigen (i.e., AG-CCA) occurred 24 to 48 h prior to isolation; AG < TCID50, isolation in conventional TC, occurred 24 to 48 h prior to antigen detection by AG-CCA; AG-CCA = TCID50, no difference to the time of viral detection by AG-CCA or isolation in conventional TCs was identified.