TABLE 1.
Distribution of agr class, core gene RFLPs, accessory gene carriage and RFLPs, 16S rRNA ribotypes and phage group among reference strains and MSSA isolatesa
| Cluster | Strain | Sourceb | agr classc | Core
genes
|
Accessory genes
|
16S rRNA ribotypee | Phage groupf | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| rnaIII | sarA | ssp | spa | icaA | fnbA | efb | hlb | hlg | cna | cap5 | cap8 | lukE | splB | tst | sea | seb | sec | sed | seg | seh | sei | ||||||
| 8325-4 | Reference | I | 2.3 + 12 | 21 | 9 | 9.4 | 8 | 2.8 | 4 + 6 | 4 | 2.1 + 6 | — | 4 | — | 6 | 17 | — | — | — | — | — | — | — | — | I | I | |
| SA502A | Reference | II | 2.3 + 7.5 | 21 | 7 | 9.4 | 8 | 3.8 | 4 + 7 | 3.5 + 7.7 | 2.1 + 6 | — | 4 | — | 3.8 | 9.4 | — | — | — | — | 4 | 8.2 | — | 8.2 | I | I | |
| RN8463 | Reference | III | 2.3 + 14 | 9.5 | 7 + 20 | 7.9 | 10 | 3.8 | 5.5 | 6 + 7.7 | 2.1 + 6 | 17 | — | 0.8 + 14 | — | — | 16 | 7.7 + 8.5 | — | — | — | 5.8 | — | 5.8 | II | I | |
| RN4850 | Reference | IV | 1 + 5.5 | 21 | 7 | 10 | 10 | 4.2 | 3.5 | 19 | 2.1 + 6 | 9.4 | — | 0.8 + 14 | 4.5 | 6 | — | — | — | — | — | 2.5 | — | 4 | V | II | |
| agr class III | PM2 | Sputum | III | 2.3 + 14 | 9.5 | 7 + 20 | 7.9 | 10 | 3.8 | 5.5 | 4.6 | 2.1 + 6 | 17 | — | 0.8 + 14 | — | — | 3 | — | — | 4 | — | — | — | — | II | I |
| PM47 | Blood culture | III | 2.3 + 14 | 9.5 | 7 + 20 | 7.9 | 10 | 3.8 | 5.5 | 9 + 19 | 2.1 + 6 | — | — | 0.8 + 14 | — | — | 16 | — | — | — | — | 5.8 | 14 | 5.8 | II | NT | |
| PM116 | Lung post mortem | III | 2.3 + 14 | 9.5 | 7 + 20 | 7.9 | 10 | 3.8 | 5.5 | 6 + 7.7 | 2.1 + 6 | 17 | — | 0.8 + 14 | — | — | 16 | 7.7 + 8.5 | — | — | — | 5.8 | — | 5.8 | II | I | |
| PM122 | Nose | III | 2.3 + 14 | 9.5 | 7 + 20 | 7.9 | 10 | 3.8 | 5.5 | 9 | 2.1 + 6 | 17 | — | 0.8 + 14 | — | — | 16 | — | — | — | — | 5.8 | — | 5.8 | II | I | |
| PM37 | Blood culture | III | 2.3 + 12 | 9.5 | 7 + 20 | 7.9 | 10 | 3.8 | 5.5 | 9 + 19 | 2.1 + 6 | — | — | 0.8 + 14 | — | — | 16 | — | — | — | — | 5.8 | 14 | 5.8 | II | I | |
| PM58 | Sputum | III | 2.3 + 12 | 9.5 | 7 + 20 | 7.9 | 10 | 3.8 | 5.5 | 4.6 | 2.1 + 6 | 17 | — | 0.8 + 14 | — | — | 6 | — | — | 4 | — | 5.8 | — | 5.8 | II | I | |
| SGH-1 | PM13 | Blood culture | I | 2.3 + 7.5 | 17 | 5 | 10 | 15 | 3.8 | 3.5 | 7.7 | 1.2 + 6 | 9.4 | — | 0.8 + 14 | — | — | — | — | — | — | — | 9.4 | — | 9.4 | VII | I |
| PM35 | Blood culture | I | 2.3 + 7.5 | 17 | 5 | 10 | 15 | 3.8 | 3.5 | 6 | 1.2 + 6 | 9.4 | — | 0.8 + 14 | — | — | — | — | — | 4 | — | 9.4 | — | 9.4 | VII | I | |
| PM52 | Blood culture | I | 2.3 + 7.5 | 17 | 5 | 10 | 15 | 3.8 | 3.5 | 6 | 1.2 + 6 | 9.4 | — | 0.8 + 14 | — | — | — | — | 5.5 | — | — | 9.4 | — | 9.4 | VII | I | |
| PM54 | Blood culture | I | 2.3 + 7.5 | 17 | 5 | 10 | 15 | 3.8 | 3.5 | 6 | 1.2 + 6 | 9.4 | — | 0.8 + 14 | — | — | — | — | 5.5 | — | — | 9.4 | — | 9.4 | VII | I | |
| PM83 | Blood culture | I | 2.3 + 7.5 | 17 | 5 | 10 | 15 | 3.8 | 3.5 | 6 | 1.2 + 6 | 9.4 | — | 0.8 + 14 | — | — | — | — | — | — | — | 9.4 | — | 9.4 | VII | I | |
| PM127 | Sputum | I | 2.3 + 7.5 | 17 | 5 | 10 | 15 | 3.8 | 3.5 | 6 | 1.2 + 6 | 9.4 | — | 0.8 + 14 | — | — | — | — | — | 4 | — | 9.4 | — | 9.4 | VII | I | |
| PM94 | Nose | I | 2.3 + 7.5 | 17 | 5 + 7 | 10 | 15 | 3.8 | 3.5 | 6 | 1.2 + 6 | 9.4 | — | 0.8 + 14 | 3.6 | 9.4 | — | — | — | 4 | — | 14 | — | 14 | VII | NT | |
| SGH-2 | PM20 | Blood culture | I | 2.3 + 12 | 21 | 9 | 9.4 | 8 | 2.8 | 4 + 6 | 4 + 7.3 + 7.7 | 2.1 + 6 | — | 4 | — | 6 | 17 | — | 2.5 + 6.7 | — | — | 4 | — | — | — | I | I |
| PM23 | Blood culture | I | 2.3 + 12 | 21 | 9 | 9.4 | 8 | 2.8 | 5.5 | 6 + 21 | 2.1 + 6 | — | 4 | — | 6 | 17 | — | — | — | — | — | — | — | — | I | I | |
| PM80 | Blood culture | I | 2.3 + 12 | 21 | 9 | 9.4 | 8 | 2.8 | 4 + 6 | 7 + 7.7 | 2.1 + 6 | — | 4 | — | 6 | 17 | — | 2.5 + 6.7 | — | — | 4 | — | — | — | I | I | |
| PM82 | Blood culture | I | 2.3 + 12 | 21 | 9 | 9.4 | 8 | 2.8 | 4 + 6 | 6 + 19 | 2.1 + 6 | — | 4 | — | 6 | 17 | — | 22 | — | — | — | — | —— | — | I | III | |
| PM118 | Sputum | I | 2.3 + 12 | 21 | 9 | 9.4 | 8 | 2.8 | 4 + 6 | 6 + 19 | 2.1 + 6 | — | 4 | — | 6 | 17 | — | — | — | — | — | — | — | — | I | I | |
| PM146 | Lung postmortem | I | 2.3 + 12 | 21 | 9 | 9.4 | 8 | 2.8 | 4 + 6 | 4 | 2.1 + 6 | — | 4 | — | 6 | 17 | — | — | — | — | — | — | — | — | I | NT | |
| SGH-3 | PM12 | Blood culture | II | 2.3 + 7.5 | 21 | 7 | 6.6 | 8 | 2.8 + 3.8 | 4 + 6 | 3.5 + 4 | 2.1 + 6 | — | — | 0.8 + 14 | 3.8 | 9.4 | — | — | — | — | — | — | — | — | I | II |
| PM149 | Sputum | II | 2.3 + 7.5 | 21 | 7 | 6.6 | 8 | 2.8 + 3.8 | 4 + 6 | 3.5 + 4 | 2.1 + 6 | — | — | 0.8 + 14 | 3.8 | 9.4 | — | — | — | — | — | — | — | — | I | II | |
| PM153 | Throat Swab | II | 2.3 + 7.5 | 21 | 7 | 6.6 | 8 | 2.8 + 3.8 | 4 + 6 | 3.5 + 4 | 2.1 + 6 | — | — | 0.8 + 14 | 3.8 | 9.4 | — | — | — | — | — | — | — | — | I | II | |
| PM154 | Lung postmortem | II | 2.3 + 7.5 | 21 | 7 | 6.6 | 8 | 2.8 + 3.8 | 4 + 6 | 3.5 + 4 | 2.1 + 6 | — | — | 0.8 + 14 | 3.8 | 9.4 | — | — | — | — | — | — | — | — | I | II | |
| PM19 | Blood culture | II | 2.3 + 7.5 | 21 | 7 | 6.6 | 8 | 2.8 | 4 + 6 | 3.5 + 4 | 2.1 + 6 | — | — | 0.8 + 14 | 3.8 | 9.4 | — | — | — | — | — | — | — | — | I | II | |
| PM131 | Nose | II | 2.3 + 7.5 | 21 | 7 | 6.6 | 8 | 2.8 | 4 + 6 | 3.5 + 4 | 2.1 + 6 | — | — | 0.8 + 14 | 3.8 | 9.4 | — | — | — | — | — | — | — | — | I | II | |
| PM148 | Sputum | II | 2.3 + 7.5 | 21 | 7 | 6.6 | 8 | 2.8 | 4 + 6 | 3.5 + 4 | 2.1 + 6 | — | — | 0.8 + 14 | 3.8 | 9.4 | — | — | — | — | — | — | — | — | I | II | |
| PM45 | Blood culture | I | 2.3 + 7.5 | 21 | 7 | 9.4 | 8 | 3.8 | 4 | 7 + 7.7 | 2.1 + 6 | 9.4 | — | 0.8 + 14 | 3 | 17 | — | 2.5 + 6.7 | — | — | — | — | — | — | I | NT | |
| PM46 | Blood culture | I | 2.3 + 7.5 | 21 | 7 | 9.4 | 8 | 3.8 | 4 | 7 + 7.7 | 2.1 + 6 | 9.4 | — | 0.8 + 14 | 3 | 17 | — | 2.5 + 6.7 | — | — | — | — | — | — | I | NT | |
| PM99 | Sternal wound swab | I | 2.3 + 7.5 | 21 | 7 | 9.4 | 8 | 3.8 | 4 | 3 + 19 | 2.1 + 6 | 9.4 | 4 | — | 3.8 | 17 | — | — | — | — | — | 8.2 | — | 8.2 | I | V | |
| PM126 | Sputum | I | 2.3 + 7.5 | 21 | 7 | 9.4 | 8 | 3.8 | 4 | 19 | 2.1 + 6 | — | 4 | — | 3.6 | 17 | — | — | — | — | — | 8.2 | — | 8.2 | I | I | |
| PM50 | Blood culture | I | 2.3 + 7.5 | 21 | 7 | 9.4 | 8 | 3.8 + 5 | 4 | 7 + 7.7 | 2.1 + 6 | 9.4 | — | 0.8 + 14 | 3 | 17 | — | 2.5 + 6.7 | — | — | — | — | — | — | I | III | |
| PM139 | Hip tissue | I | 2.3 + 7.5 | 21 | 7 | 9.4 | 8 | 3.8 + 5 | 4 | 9 | 2.1 + 6 | 9.4 | — | — | 3 | 17 | — | — | — | — | — | — | — | — | VIII | III | |
| PM26 | Blood culture | I | 2.3 + 7.5 | 21 | 7 | 9.4 | 8 | 10 | 3.8 | 7.7 + 19 | 2.1 + 6 | 9.4 | — | 0.8 + 14 | 3.8 | 8.9 | — | — | — | — | — | — | — | — | I | II | |
| PM48 | Blood culture | I | 2.3 + 12 | 21 | 7 | 9.4 | 8 | 3.8 | 4 | 7.7 + 12 | 2.1 + 6 | — | 4 | — | 4.5 | 9.4 | — | — | — | — | — | — | — | — | I | III | |
| PM63 | Sputum | I | 2.3 + 12 | 21 | 7 | 9.4 | 8 | 10 | 4 | 12 + 19 | 2.1 + 6 | 9.4 | 4 | — | 3.8 | 8.9 | — | — | — | — | — | — | — | — | I | II | |
| PM150 | Cerebrospinal fluid | II | 2.3 + 12 | 21 | 7 | 9.4 | 8 | 3.8 | 4 + 7 | 7.7 | 2.1 + 6 | — | 4 | — | 3.8 | 9.4 | — | — | 3 | — | 4 | 8.2 | — | 8.2 | I | III | |
| PM152 | Cerebrospinal fluid | II | 2.3 + 7.5 | 21 | 7 | 9.4 | 6 | 3.8 | 3.5 | 6 | 2.1 + 6 | 9.4 | — | 0.8 + 14 | 3.8 | 9.4 | — | 22 | 3 | — | — | — | — | — | IX | III | |
| PM49 | Blood culture | I | 2.3 + 7.5 | 21 | 7 | 6.6 | 8 | 3.8 | 5.5 | 7.7 + 9 | 2.1 + 6 | — | — | 0.8 + 14 | 3.8 | 15 | — | — | — | — | — | — | — | — | VI | NT | |
| PM16 | Blood culture | I | 2.3 + 7.5 | 21 | 6.5 | 6.6 | 10 | 3.8 | 0.8 + 3.5 | 3.5 + 7.7 | 2.1 + 6 | 6 | 4 | — | — | — | — | — | — | — | — | 8.2 | — | 8.2 | X | III | |
| PM10 | Sputum | I | 2.3 + 12 | 21 | 6.5 | 6.6 | 10 | 3.8 | 3.5 | 3.5 + 7.7 | 2.1 + 6 | 6 | 4 | — | — | — | — | — | — | — | — | 8.2 | — | 8.2 | III | NT | |
| PM1 | Nose | II | 2.3 + 7.5 | 21 | 7 | 9.4 | 8 | 3.8 | 4 + 7 | 7 + 12 | 2.1 + 6 | — | 4 | — | 3.8 | 9.4 | — | 9.5 | — | — | — | 8.2 | — | 8.2 | I | I | |
| PM24 | Blood culture | II | 2.3 + 7.5 | 21 | 7 | 9.4 | 8 | 3.8 | 4 + 7 | 6 | 2.1 + 6 | — | 4 | — | 3.8 | 9.4 | — | — | — | — | — | 8.2 | — | 8.2 | I | I | |
The table summarizes the results of all experiments. The four reference strains are listed first, and then MSSA strains have been listed in cluster groups corresponding to those in Fig. 1. The following pairs of isolates were from the same patients: PM116 and PM122, PM52 and PM54, PM153 and PM154, and PM45 and PM46.
Source of each clinical isolate.
agr class was determined as described in Materials and Methods.
RFLP values are expressed as the size of ClaI fragment hybridizing to each core gene (rnaIII, sarA, ssp, spa, icaA, fnbA, efb, hlb, hlg) or accessory gene (cna, cap5, cap8, lukE, splB, tst, sea, seb, sec, sed, sed, seg, seh, sei), by Southern blotting. Numbers refer to the size of the ClaI fragment hybridizing to each probe in Southern blot (kb). When two bands hybridized to the same probe, both are listed separated by a plus sign; this occurs when there are two copies of the gene or there is a ClaI restriction site within the gene. Clusters (see Fig. 1) are indicated in the first column. —, absence of that gene.
Ribotypes were ascribed by us in this study.
Phage groups were assigned as described in Materials and Methods. NT, nontypeable by phage typing.