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. 2022 Jan 28;154(3):e202113029. doi: 10.1085/jgp.202113029

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© 2022 Ovejero et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S7. — Effect of cooling on the M2 reflection. (A and B) Axial profiles of the M2 reflection from intact trabeculae (A) and demembranated trabeculae in the presence of Dextran (B). Magenta, 39/38°C; orange, 27/26°C; green, 22°C; cyan, 16/18°C; blue, 9/7°C in intact and demembranated trabeculae, respectively. Vertical dotted and dashed-dotted line, position of the two main peaks of M2L; continuous and dashed lines, position of the two main peaks of M2H in intact trabeculae at 39°C. (C–F) M2L spacing (S_M2L; C) and total intensity (I_M2L; D) and M2H spacing (E) and total intensity (F). Gray circles and line, intact trabeculae; empty circles and dashed black line, demembranated trabeculae no Dextran; black circles and continuous line, demembranated trabeculae in the presence of Dextran. Data added from n = 5 intact trabeculae and n = 4 demembranated trabeculae. Red square in C and E, diastolic value in intact trabeculae paced at 1 Hz; T = 26.4°C; data added from n = 6 trabeculae. Intensities in D and F are normalized by I_M2H at 39/38°C in intact trabeculae and in demembranated trabeculae in the presence of Dextran. a.u., arbitrary unit.