TABLE 1.

List of oligonucleotide primers used.

Primers	Direction 5′-3′	Nucleotide sequence from 5′ to 3′	Purpose
K1	Forward	TTGCAT​ATGGGT​TTC​CAA​CCC​ACTa	Gene fragment flanking for making sarsS
K2	Reverse	GTAGAA​TTCGTT​GTT​ACA​TGT​TCA	Gene fragment flanking for making sarsS
B1	Forward	TGA​GTG​AAC​CAC​AGC​CAG​AA	Integration of the pentF-sarsS plasmid DNA into the Enterococcus in chromosomal DNA
Seq F	Forward	GGA​CAC​CAC​AAC​CAT​CGA​AG	Sequencing of the PCR product of pentF-S1
Cov1	Forward	AAGGA​TCCATA​CAT​ATG​GGT​TTC​C	Cloning a gene fragment sarsS for protein production
Cov2	Reverse	TGT​CGA​CGGAG​CTCGAA​TT	Cloning a gene fragment sarsS for protein production
A1	Forward	GCT​CTA​GAG​CCG​ATG​AGA​GCA​GCT​GGT​ATT​G	Determining the presence of inserts and a fragment of the sarsS gene in Enterococcus
D1	Reverse	CAA​CAG​GAT​CCA​AAG​CAT​CGT​TGG	Determining the presence of inserts and a fragment of the sarsS gene in Enterococcus
Dal 1	Forward	TTG​AGG​CAG​ACC​AGA​TTG​ACG	D-alanine-D-alanine ligase
Dal 2	Reverse	TAT​GAC​AGC​GAC​TCC​GAT​TCC	D-alanine-D-alanine ligase

^aThe underlined area in the nucleotide sequences correspond to restriction sites used for cloning.