TABLE 2.
Bacterial strains and plasmids used in this study.
| Strains and plasmids | Detailed information | Source |
|---|---|---|
| E. coli MG1655 | Parent strain | Lab collection |
| GXASR | E. coli MG1655/pTrc99A | This study |
| GXASR1 | E. coli MG1655/pTrc99A-budB-budA-nox | This study |
| GXASR2 | E. coli MG1655/pTrc99A-alsS-alsD-nox | This study |
| GXASR3 | E. coli MG1655 Δdar/pTrc99A-budB-budA-nox | This study |
| GXASR4 | E. coli MG1655 ΔfrdABCD/pTrc99A-budB-budA-nox | This study |
| GXASR5 | E. coli MG1655 Δdar ΔfrdABCD/pTrc99A-budB-budA-nox | This study |
| GXASR6 | E. coli MG1655 Δdar ΔfrdABCD ΔpflB/pTrc99A-budB-budA-nox | This study |
| GXASR7 | E. coli MG1655 Δdar ΔfrdABCD ΔldhA/pTrc99A-budB-budA-nox | This study |
| GXASR8 | E. coli MG1655 Δdar ΔfrdABCD Δpta/pTrc99A-budB-budA-nox | This study |
| GXASR9 | E. coli MG1655 Δdar ΔfrdABCD ΔadhE/pTrc99A-budB-budA-nox | This study |
| GXASR10 | E. coli MG1655 Δdar ΔfrdABCD ΔpflB ΔldhA/pTrc99A-budB-budA-nox | This study |
| GXASR11 | E. coli MG1655 Δdar ΔfrdABCD ΔpflB ΔldhA Δpta/pTrc99A-budB-budA-nox | This study |
| pTrc99A | Ampr | Lab collection |
| pTrc99A-budB-budA-nox | Ampr | This study |
| pTrc99A-alsS-alsD-nox | Ampr | This study |
alsD and alsS, the genes encoding α-acetolactate decarboxylase and α-acetolactate synthase from P. polymyxa, respectively.