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. 2001 Aug;39(8):2788–2793. doi: 10.1128/JCM.39.8.2788-2793.2001

FIG. 2.

FIG. 2

Western immunoblot analysis of sera from dog 35 (military dog, 1:160 E. canis IFA titer) (A), dog 12 (civilian dog, 1:80 E. canis IFA titer) (B), dog 33 (military dog, 1:160 E. canis IFA titer) (C), dog 10184 experimentally infected with E. canis Oklahoma (1:5,120 E. canis IFA titer) (D), dog 30133 experimentally infected with E. chaffeensis Arkansas (1:640 E. chaffeensis IFA titer) (E), and PCR-confirmed human patient (patient 42) infected with E. chaffeensis (1:1,280 E. chaffeensis IFA titer) (F). Lanes: DH82, dog macrophage cell line DH82 (negative control); C, pET29-transformed E. coli (negative control); VDE, purified VDE; VHE, purified VHE; Eca, purified E. canis; Ech, purified E. chaffeensis; Eca, rP30, affinity-purified recombinant fusion protein of E. canis (27 kDa). The numbers on the left of each panel indicate molecular masses in kilodaltons based on the broad-range prestained standards (Bio-Rad). The arrowheads show the position of rP30.