TABLE 1.
Sequences of the primers and probes used in the dengue NASBA assays
Primer or probe | Sequencea | Genome positionb (GenBank accession no.) |
---|---|---|
P1 primer | 5′AATTCTAATACGACTCACTATAGGGGAGACAGCAGGATCTCTGGTCT3′ | 10,632–10,653 (M87512) |
P2 primer | 5′GATGCAAGGTCGCATATGAGGGTTAGAGGAGACCCCTCCC3′ | 10,497–10,516 (M87512) |
Ru2+-labeled detector probe | 5′GATGCAAGGTCGCATATGAG3′ | |
Type 1 capture probe | 5′GGGAAGCTGTATCCTGGTGGTAAGG3′ | 10,550–10,574 (M87512) |
Type 2 capture probe | 5′ATGAAGCTGTAGTCTCACTGGAAGG3′ | 10,557–10,581 (M20558) |
Type 3 capture probe | 5′AGGGAAGCTGTACCTCCTTGCAAAG3′ | 10,530–10,554 (M93130) |
Type 4 capture probe | 5′GAGGAAGCTGTACTCCTGGTGGAAG3′ | 10,483–10,507 (M14931) |
Conserved capture probe | 5′AAACAGCATATTGACGCTGGG3′ | 10,609–10,629 (M87512) |
Italicized bases represent overhang sequences of the primers: a T7 promoter sequence on P1, and a sequence homologous to the detector probe on P2.
In bases.