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(a) Schematic of the laser induced forward transfer (LIFT) system used for single-microorganism isolating and culturing. L1 to L4, lenses; HP, half-waveplate; PBS, polarizing beam splitter; S, Shutter; M1 to M4, mirrors; DM1, dichroic mirror; MO1 to MO2, microscopy objectives. (b) Three-layer structure. 1st layer, Al film; 2nd layer, solid culture medium; 3rd layer, liquid culture medium.
