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. 2022 Feb 7;14(1):2035658. doi: 10.1080/19490976.2022.2035658

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© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Effects of delayed fecal sample processing on the cultivability of microbiomes. (a) Principal component analysis (PCA) score plots of quantified protein groups. Baseline, uncultured microbiome samples; FOS, fructo-oligosaccharide treatment group; PBS, phosphate buffered saline treatment group. V31, V33 and V34, origin donors of the microbiome samples; Fresh, culturing with fresh stools; ICE6h, culturing with stools stored on ice for 6 hours; DRYICE6h, culturing with stools stored on dry ice for 6 hours. (b) Phylum level taxonomic compositions as calculated using metaproteomic data. Group average of relative abundances was used for plotting. NA indicated no treatment/culturing for baseline samples. Different colors indicate different phyla as shown in the legend at the bottom.