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. 2022 Feb 6;13(1):355–369. doi: 10.1080/21505594.2022.2032928

Figure 1.

Figure 1.

MERS-CoV nsp1 inhibits cell viability through its endonuclease activity. (a) HEK293T cells were transfected with MERS-CoV nsp overexpression vectors, and a CCK8 assay was performed 48 h after transfection. (b) The expression of nsp1 and β-actin was determined by Western blot in HEK293T cells. (c-d) Analysis of the cell cycle in HEK293T cells. (c) Flow cytometry analysis of the cell cycle in HEK293T cells transfected with nsp1 for 36 h. (d) Quantification of flow cytometry cell cycle analysis of nsp1. (e) The effects of MERS-CoV nsp1 on wound healing in HEK293T cells. (f) Quantification of wound closure rate. (g-l) HEK293T cells were transfected with MERS-CoV nsp1, nsp1-CD, or empty vector. (g) The expression of nsp1/nsp1-CD and β-actin was determined by Western blot in HEK293T cells. (h) A CCK8 assay was performed at the indicated time points. (i) Flow cytometry analysis of the cell cycle in HEK293T cells 36 h post-transfection. (j) Quantification of flow cytometry cell cycle analysis of nsp1 and nsp1-CD, at 36 h post ORF introduction. (k) Wound healing of HEK293T cells. (l) Quantification of wound closure rate. Data are mean ± s.d. from at least three independent experiments. *p < 0.05, **p < 0.01, ****p < 0.001.