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. 2022 Feb 6;13(1):355–369. doi: 10.1080/21505594.2022.2032928

Figure 3.

Figure 3.

MERS-CoV nsp1 decreases ribosome number and disturbs mitochondrial function. (a) Ribosomes (right) and mitochondria (left) in HEK293T cells transfected with control vector, MERS-CoV nsp1, or nsp1-CD were assessed by electron microscopy. (b) The expression of nsp1 and β-actin in A549 cells was determined by Western blot. (c) Confocal microscopy of Mito-Tracker-Red in A549 cells transfected with control vector, MERS-CoV nsp1, and nsp1-CD. Nuclei were stained with DAPI. The images are representatives of at least three independent experiments. (d) Oxygen consumption rates (OCR) were measured in control vector-, MERS-CoV nsp1-, nsp1-CD-transfected A549 cells treated with oligomycin (1.5 μM), FCCP (1 μM), Rot & AA (0.5 μM). (e-g) Quantification of OCR-based basal respiration, ATP production, and spare respiration capacity analysis of control vector, nsp1, and nsp1-CD- transfected A549 cells. Data are mean ± s.d. from at three independent experiments. *p < 0.05, **p < 0.01.