Fig. 6.

Various concentrations (12.5 μg/ml, 25 μg/ml, and 50 μg/ml) of aristolactam AII treated A549 cells for 24 h suppressed CDKs, cyclins, Bcl-2 protein expression and up-regulation of apoptotic protein (caspase 3, caspase 8, p21, p27, p53, and Bax) expression (A). Protein/β actin level (%) presented in (B). The experiments were done in triplicate with similar results and used the blot of β actin as a loading control. *P < 0.05, **P < 0.01, ***P < 0.001 was considered statistically significant compared to the control group.