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The primer sequences for cDNA synthesis and quantitative real-time PCR (qRT-PCR).
| miRBase accession no. | Primer name | Sequence(5′3′) |
|---|---|---|
| MIMAT0000082 | has-miR-26a-5p-RT1 | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGCCT |
| has-miR-26a-5p-Forward | CGCCGCTTCAAGTAATCCAGG | |
| MIMAT0000069 | has-miR-16-5p-RT1 | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCGCCAAT |
| has-miR-16-5p-Forward | GGTAGCAGCACGTAAATATTGGCG | |
| Universal- reverse (UR) | GTGCAGGGTCCGAGGT |
Stem-loop primer for cDNA synthesis
