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. 2022 Jan 27;20(1):e3001532. doi: 10.1371/journal.pbio.3001532

Fig 2. Intestinal inflammation elicits a coordinated transcriptional response in the vascular endothelium.

Fig 2

(A) Single-cell atlas of colon endothelial cells. UMAP of endothelial cell (dots) profiles colored by cell type assignment. (B) Expression of canonical markers across endothelial cell clusters. Color represents average expression of marker gene within clusters; diameter represents percentage expression of marker gene within cluster. (C) Volcano plots depicting DEGs between water- and DSS-treated samples for select endothelial cell clusters. Each dot represents an individual gene, and dot colors represent statistical significance. Gray, not differentially expressed; orange, differentially expressed but gene name is not annotated; blue, differentially expressed and gene name is annotated. Significant DEGs had FDR <0.05 using MAST (see Methods). (D-F) Violin plots of expression levels of select novel (D), chemokine (E), and adhesion (F) transcripts across endothelial cell clusters in water- and DSS-treated samples. Normalized gene expression levels are plotted on the y-axis. Significant DEGs had FDR <0.05, and respective adjusted p-values derived using MAST (see Methods); *p < 0.05, **p < 0.001, ***p < 1E-10. DEG, differentially expressed gene; DSS, dextran sulfate sodium; FDR, false discovery rate; LEC, lymphatic endothelial cell; UMAP, uniform manifold approximation and projection.