Figure 6. Cx43(M1) inhibits the load-induced increase in midshaft endosteal osteogenesis.

Dynamic histomorphometric analyses were performed on the tibial midshaft cortical endosteal (a–d) and periosteal (e–h) surfaces after 2 weeks of loading in vehicle and Cx43(M1)-treated mice. (a, e) Representative images of calcein (green) alizarin (red) double labeling on (a) endosteal and (e) periosteal surface Scale bar: 50 μm. Mineral apposition rate (MAR), mineralizing surface/bone surface (MS/BS), and bone formation rate (BFR/BS) were assessed for (b–d) endosteal and (f–h) periosteal surfaces (n = 6/group). Data are expressed as mean ± SD. ^*, p < 0.05; ^**, p < 0.01; ^***, p < 0.001. Statistical analysis was performed using paired t-test for loaded and contralateral within each treatment.