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. 2022 Feb 9;42(6):1068–1089. doi: 10.1523/JNEUROSCI.1724-21.2021

Figure 3.

Figure 3.

Chemoarchitecture of the reuniens nucleus. A–C, Brightfield photomicrographs of coronal sections immunolabeled for CB through the ventral midline thalamic nuclei. Dashed line denotes midline, and black arrowheads point to the RE. Right, bottom, Plane of section through thalamus (Th). D, Inset, From B, black arrows indicate darkly labeled CB neurons. E–G, Brightfield photomicrographs of coronal sections immunolabeled for PV through the ventral midline thalamic nuclei. H, Inset, From E, black arrow indicates darkly labeled PV cell. The surrounding area contains PV+ labeled processes, some of which are likely axons from the TRN. I–K, Brightfield photomicrographs of coronal sections immunolabeled for CR (brown) and counterstained for Nissl (blue) through the ventral midline thalamic nuclei. L, Inset, From I, black arrows indicate darkly labeled CR neurons. M–O, Brightfield photomicrographs of coronal sections immunolabeled for GABA through the ventral midline thalamic nuclei. P, Inset, From N, black arrows indicate darkly labeled GABA cells. Q, Collapsed confocal microscopy z stack (maximum projection) shows tracer-labeled axon terminations (green; here, BQ-Alexa488 injected in hippocampus, as in R, S. comingling with PV-positive neuronal processes (red) in RE. Empty white arrowhead indicates a retrogradely labeled neuron. Scale bar applies to R, S. R, Collapsed z stack shows tracer-labeled axon terminations from hippocampus (green) comingling with CB-positive neurons and processes (red) in RE. White arrowhead indicates a CB-positive retrogradely labeled neuron (small green-yellow granules indicate neural tracer colabeling). White arrow indicates darkly labeled CB neuron. S, Collapsed z stack shows tracer-labeled axon terminations from hippocampus (green) comingling with CR-positive neurons and processes (red) in the RE. White arrowhead indicates a CR-positive retrogradely labeled neuron. White arrow indicates darkly labeled CR neuron. T, Electron photomicrograph of a PV-positive dendrite in RE immunolabeled with TMB (black stripe, black arrow). An unlabeled axon terminal forms an asymmetric (excitatory) synapse along the lower right aspect of the dendrite. Scale bar applies to U-W. U, Electron photomicrograph of a CB-positive dendrite in RE immunolabeled with gold (round black clusters, black arrow). An unlabeled axon terminal forms a perforated asymmetric (excitatory) synapse along the lower aspect of the dendrite. V, Electron photomicrograph of a CR-positive dendrite in RE immunolabeled with TMB (black smears, black arrows). An unlabeled axon terminal forms an asymmetric (excitatory) synapse along the lower left aspect of the dendrite W, Electron photomicrograph of a GAD67-positive dendrite in RE immunolabeled with gold (round black clusters, black arrow). C, Caudal; D, dorsal; Hipp, hippocampus; III, third ventricle; R, rostral; Th, thalamus; V, ventral.