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. 2022 Feb 9;42(6):1068–1089. doi: 10.1523/JNEUROSCI.1724-21.2021

Table 1.

Case, injection site, tracer used, analysis performed

Case Hemisphere, sex, age Injection site used Tracer Analysis
1 AJ L + R, F n/a n/a Photography of Nissl-stained sections
2 AZ L + R, M n/a n/a Photography and plotting of CB and PV neurons
3 BB L, F, 2 y Left-Amy (BM, BL, Ant)
Right-Amy (BL, Ant, BM, Co)
BDA (3 kDA) Bouton measurement (OM, L hemisphere)
4 BD L + R, M, 2.5 y Left-Amy (BL, Ce, La, BM)
Right-Amy (Co, Me, BM, BL)
BDA Bouton measurement (OM, L hemisphere), plotting of CB and PV neurons (R hemisphere)
5 BM R, F, 3.5 y Amy (BL) FR (10 + 3 kDA) Axon mapping, stereologic axon termination quantification, bouton measurement (OM), synaptic analysis (EM)
6 BQ L, F, 3.5 y Hipp (DG, CA1, ProS) Alexa488 (10 kDA) Axon mapping, stereologic axon termination quantification, bouton measurement (OM), synaptic analysis (EM), confocal photography
BQ L A25 (anterior medial) FR (10 + 3 kDA) Bouton measurement (OM)
BQ R Amy (BM and BL) CBL, (10 + 3 kDA) Synaptic analysis (EM)
7 BR L, F, 3 y A25 (posterior medial) BDA (10 + 3 kDA) Axon mapping, stereologic axon termination quantification, bouton measurement (OM), synaptic analysis (EM)
8 BS L, F, 3.5 y A25 (anterior medial) LY (10 kDA) Bouton measurement (OM), synaptic analysis (EM)
BS R Hipp (CA1) CBL (10 + 3 kDA) Bouton measurement (OM)
9 BT L, F, 4 y Hipp (DG, CA1, CA3) FE (10 + 3 kDA) Axon mapping, stereologic axon termination quantification, bouton measurement (OM)
BT R Amy (BL, BM) BDA (10 + 3 kDA) Axon mapping, stereologic axon termination quantification, bouton measurement (OM)
10 BU L, M, 4 y Hipp (DG, CA3, CA2, CA1) CBL (10 + 3 kDA) Synaptic analysis (EM)
BU R A25 (anterior orbital) FE (10 + 3 kDA) Axon mapping, stereologic axon termination quantification, bouton measurement (OM)
11 BV R, M, 4 y n/a n/a Photography and plotting of CR neurons, photography and plotting of GABA neurons
12 BW R, F, 5.5 y Amy (BM, BL, Co) FR (10 + 3 kDA) Synaptic analysis (EM), plotting of CR neurons, photography and plotting of GABA neurons

Amy, Amygdala; BL, basolateral nucleus of the amygdala; BM, basomedial nucleus of the amygdala; CA, cornu ammonis; CBL, Cascade Blue; Ce, central nucleus of the amygdala; Co, cortical nucleus of the amygdala; DG, dentate gyrus; F, female; FE, Fluoroemerald; FR, Fluororuby; Hipp, hippocampus; kDA, kilodalton; L, left; La, lateral nucleus of the amygdala; LY, Lucifer yellow; Me, medial nucleus of the amygdala; n/a, not applicable; OM, optical microscopy; ProS, prosubiculum; R, right; y, year.