Table 1.
Case | Hemisphere, sex, age | Injection site used | Tracer | Analysis | |
---|---|---|---|---|---|
1 | AJ | L + R, F | n/a | n/a | Photography of Nissl-stained sections |
2 | AZ | L + R, M | n/a | n/a | Photography and plotting of CB and PV neurons |
3 | BB | L, F, 2 y | Left-Amy (BM, BL, Ant) Right-Amy (BL, Ant, BM, Co) |
BDA (3 kDA) | Bouton measurement (OM, L hemisphere) |
4 | BD | L + R, M, 2.5 y | Left-Amy (BL, Ce, La, BM) Right-Amy (Co, Me, BM, BL) |
BDA | Bouton measurement (OM, L hemisphere), plotting of CB and PV neurons (R hemisphere) |
5 | BM | R, F, 3.5 y | Amy (BL) | FR (10 + 3 kDA) | Axon mapping, stereologic axon termination quantification, bouton measurement (OM), synaptic analysis (EM) |
6 | BQ | L, F, 3.5 y | Hipp (DG, CA1, ProS) | Alexa488 (10 kDA) | Axon mapping, stereologic axon termination quantification, bouton measurement (OM), synaptic analysis (EM), confocal photography |
BQ | L | A25 (anterior medial) | FR (10 + 3 kDA) | Bouton measurement (OM) | |
BQ | R | Amy (BM and BL) | CBL, (10 + 3 kDA) | Synaptic analysis (EM) | |
7 | BR | L, F, 3 y | A25 (posterior medial) | BDA (10 + 3 kDA) | Axon mapping, stereologic axon termination quantification, bouton measurement (OM), synaptic analysis (EM) |
8 | BS | L, F, 3.5 y | A25 (anterior medial) | LY (10 kDA) | Bouton measurement (OM), synaptic analysis (EM) |
BS | R | Hipp (CA1) | CBL (10 + 3 kDA) | Bouton measurement (OM) | |
9 | BT | L, F, 4 y | Hipp (DG, CA1, CA3) | FE (10 + 3 kDA) | Axon mapping, stereologic axon termination quantification, bouton measurement (OM) |
BT | R | Amy (BL, BM) | BDA (10 + 3 kDA) | Axon mapping, stereologic axon termination quantification, bouton measurement (OM) | |
10 | BU | L, M, 4 y | Hipp (DG, CA3, CA2, CA1) | CBL (10 + 3 kDA) | Synaptic analysis (EM) |
BU | R | A25 (anterior orbital) | FE (10 + 3 kDA) | Axon mapping, stereologic axon termination quantification, bouton measurement (OM) | |
11 | BV | R, M, 4 y | n/a | n/a | Photography and plotting of CR neurons, photography and plotting of GABA neurons |
12 | BW | R, F, 5.5 y | Amy (BM, BL, Co) | FR (10 + 3 kDA) | Synaptic analysis (EM), plotting of CR neurons, photography and plotting of GABA neurons |
Amy, Amygdala; BL, basolateral nucleus of the amygdala; BM, basomedial nucleus of the amygdala; CA, cornu ammonis; CBL, Cascade Blue; Ce, central nucleus of the amygdala; Co, cortical nucleus of the amygdala; DG, dentate gyrus; F, female; FE, Fluoroemerald; FR, Fluororuby; Hipp, hippocampus; kDA, kilodalton; L, left; La, lateral nucleus of the amygdala; LY, Lucifer yellow; Me, medial nucleus of the amygdala; n/a, not applicable; OM, optical microscopy; ProS, prosubiculum; R, right; y, year.