Figure 2.
Hemigrapsus sanguineus. Variations in the haemolymph osmolality in different life cycle stages in relation to the osmolality (bottom x axis) and salinity (upper x axis) of the medium at 24°C. Acclimation time was 24 h for larval and crab I stages, and 6 days for adults. Values are shown as average values ± standard error. For zoeal stages I–III: n = 9–10, IV–V: n = 5; for megalopa: n = 6–8, for crab I: n = 4–5; for adults: n = 9–12, except for crabs exposed to < 1 ppt: n = 1, 5 ppt: n = 3 and 10 ppt: n = 6. Zoeal stages are shown in red (Z I: stars, Z II: diamonds, Z III: triangles, Z IV: squares and Z V: circles); megalopa (M) is shown in green squares; first juvenile crab (C I) is shown in light blue circles and adult is shown in dark blue circles. Note that standard errors may be smaller than symbols.