Figure 2.

Therapeutic efficacy of TSL-Dox and HT in B16F10 tumour-bearing mice. Growth curves B16F10 tumours treated with TSL-Dox and (A) one HT or (B) two HT. Survival curves of B16F10 tumour-bearing mice treated with TSL-Dox and (C) one HT or (D) two HT. B16F10 cells (2.5x10⁵) were injected subcutaneously in the left leg, and therapy started on day 7 with tumour size of 50-60 mm³. Animals were injected intravenously with 5 mg Dox/kg of liposome-Dox formulations (LTSL-Dox, TTSL-Dox, NTSL-Dox) followed by 60 min HT by immersing the tumour-bearing leg into 43 °C water bath. HT was applied immediately after injection, and mice were subjected to second HT (60 min) 24 hr post the first HT. Control animals were treated with HT with no Dox injection. Each group (n=5-6, error bars: ± S.E.M). For the tumour growth study, statistical analysis was performed using one-way ANOVA followed by Bonferroni post-test. * denotes comparison between control and the treatments (* p < 0.05, ** p < 0.01, *** p < 0.001).