Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 4;15:761–773. doi: 10.2147/JIR.S350421

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 Yang et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

Next-Generation Sequencing (NGS) validation with real-time PCR. From the 72 genes that demonstrated recovery to baseline levels after LVA, gene expressions with fold changes greater than two were selected for NGS validation with real-time PCR. GPI, SPYR1, SLC1A3, ZBTB16, ALOX15B, and CCL2 with gene expression ranging from 2.3- to 8.8-fold changes were selected and analyzed by real-time PCR. The results were (A) GPI was an upregulated gene before LVA and remained upregulated after LVA; (B–E) SPYR1, SLC1A3, ZBTB16, and ALOX15B showed significantly upregulated expressions before LVA and were restored to baseline expression after LVA; (F) CCL2 was down-regulated before LVA but demonstrated no significant post-LVA change. These findings validated the NGS data. *In the figure represented statistical significant difference.