FIG. 2.

(A and B) Detection limits for IHHNV (A) and WSV (B) DNAs by conventional PCR. The dilutions of genomic DNAs of IHHNV- and WSV-infected P. stylirostris samples were amplified using virus-specific primers. (C and D) β-Actin DNA was amplified from each sample in parallel. The sizes of the amplicons were 329, 306, and 339 bp for IHHNV, WSV, and the β-actin gene, respectively. Lanes 1 to 9 contain 100 ng, 10 ng, 1 ng, 100 pg, 10 pg, 1 pg, 100 fg, 10 fg, and 1 fg, respectively. Lane M, 50-bp DNA step ladder (Sigma); lane −ve, negative control.