TABLE 1.

Details of the primers used for the amplification of IHHNV, WSV, and the β-actin gene of shrimp by conventional PCR

Virus	Primer name	Primer sequence (5′-3′)	% GC	Tm (°C)a	Size (bp)	Reference
IHHNV	1641F	AGAACAATGATCCGGTCACAAGTCA	44	63	1,368	24
	1148R	AACACGCCGAAGGATCAA	50	56
	IHHN-aFor	GTACATGGTGCGTGA	53	37	329	24
	607R	AGCCTATACAATCCATGGTGACACT	44	51
WSV	F002	GATGAGACAGCCCAAGTTGTTAAAC	44	51	306	13
	R002	CGAAATTCCATCACTGTAATTGCTTG	38	50
β-Actin	Actin-F	CCCAGAGCAAGAGAGGTA	55	45	339	4, 7, 21
	Actin-R3	GCGTATCCTTCGTAGATGGG	55	49

^aT_m at 50 mM Na⁺.