TABLE 2.

List of primers used for the detection of IHHNV, WSV, and the β-actin gene of shrimp by SYBR Green PCR

Virus or gene	Primer name	SYBR Green primer sequence (5′-3′)	% GC	Tma	Amplicon size (bp)
IHHNV	313F	Forward: AGGAGACAACCGACGACATCA	52	49	50
	363F	Reverse: CGATTTCCATTGCTTCCATGA	42	45
WSV	101-110F	Forward: GATAAGAGAGGTAGACACTAGTAGTGTTATTGCT	38	56	55
	101-165R	Reverse: CCACTGTGCCAGCTATTGCA	55	49
β-Actin gene	178F	Forward: GGTCGGTATGGGTCAGAAGGA	57	51	50
	228R	Reverse: TTGCTTTGGGCCTCATCAC	52	46

^aT_m at 50 mM Na⁺.