. 2001 Aug;39(8):2835–2845. doi: 10.1128/JCM.39.8.2835-2845.2001

TABLE 3.

C_T values of replicate assays of dilution series of IHHNV and WSV plasmid standards and genomic DNA of virus-infected P. stylirostris

Virus	No. of copies of plasmid or genomic DNA or β-actin control	C_T values^a				Mean	SD	CV^b
Virus	No. of copies of plasmid or genomic DNA or β-actin control	Expt 1	Expt 2	Expt 3	Expt 4	Mean	SD	CV^b
IHHNV	Plasmid copy no.
	1.88 × 10⁵	21.253	22.350	22.185	23.010	22.200	0.725	3.27
	1.88 × 10⁴	24.220	25.700	25.315	25.115	25.088	0.627	2.49
	1.88 × 10³	28.443	30.625	28.785	28.625	29.120	1.013	3.49
	1.88 × 10²	32.730	32.445	32.980	NT	32.718	0.268	0.82
	1.88 × 10¹	35.060	35.560	35.515	35.965	35.525	0.370	1.04
	9.4	37.307	37.270	36.390	36.610	36.894	0.464	1.26
	4.7	39.130	40.000	38.635	39.620	39.346	0.593	1.51
	2.35	38.457	38.910	38.175	39.220	38.690	0.465	1.20
	1.18	39.883	40.000	40.000	39.120	39.751	0.424	1.07
	Genomic DNA sample
	1	32.983	32.187	32.335	NT	32.502	0.424	1.31
	2	34.280	34.795	34.530	NT	34.535	0.258	0.75
	3	33.935	33.747	33.545	NT	33.742	0.195	0.58
	4	31.620	32.065	32.600	NT	32.095	0.491	1.53
	5	33.627	33.345	32.190	NT	33.054	0.761	2.30
	6	33.530	34.860	33.307	NT	33.899	0.840	2.48
	7	30.873	31.305	30.730	NT	30.969	0.299	0.97
	8	31.860	32.075	30.767	NT	31.567	0.702	2.22
	9	31.697	33.005	31.480	NT	32.061	0.825	2.57
	10	30.917	31.825	30.745	NT	31.162	0.580	1.86
	β-Actin control
	1	23.567	23.893	24.035	NT	23.832	0.240	1.01
	2	24.755	26.030	25.580	NT	25.455	0.647	2.54
	3	24.823	25.477	25.435	NT	25.245	0.366	1.45
	4	NT	24.470	24.775	NT	24.623	0.216	0.88
	5	25.397	26.170	26.045	NT	25.871	0.415	1.60
	6	20.460	21.690	21.020	NT	21.057	0.616	2.93
	7	20.033	20.995	20.595	NT	20.541	0.483	2.35
	8	22.595	22.230	22.070	NT	22.298	0.269	1.21
	9	22.575	22.895	22.470	NT	22.647	0.221	0.98
	10	22.210	22.895	22.510	NT	22.538	0.343	1.52
WSV	Plasmid copy no.
	2.37 × 10⁵	21.260	21.287	21.327	21.240	21.278	0.037	0.17
	2.37 × 10⁴	25.143	24.897	25.057	25.437	25.133	0.227	0.90
	2.37 × 10³	28.860	28.987	28.455	29.373	28.919	0.378	1.31
	2.37 × 10²	32.863	32.787	32.247	32.570	32.617	0.276	0.85
	2.37 × 10¹	35.280	36.307	35.770	35.867	35.806	0.421	1.18
	11.85	36.717	36.600	37.737	37.310	37.091	0.531	1.43
	5.93	38.057	38.053	37.837	38.270	38.054	0.177	0.47
	2.96	38.690	36.670	37.653	37.667	37.670	0.825	2.19
	1.48	39.653	38.677	38.463	39.130	38.981	0.528	1.35
	Genomic DNA sample
	1	26.023	25.047	25.537	NT	25.536	0.399	1.56
	2	24.007	24.007	24.227	NT	24.080	0.104	0.43
	3	23.983	23.770	23.360	NT	23.704	0.259	1.09
	4	22.867	22.857	23.177	NT	22.967	0.149	0.65
	5	22.167	22.020	22.267	NT	22.151	0.101	0.46
	6	25.857	26.223	26.040	NT	26.040	0.150	0.58
	7	23.960	23.800	23.493	NT	23.751	0.113	0.48
	8	23.007	22.843	22.837	NT	22.896	0.115	0.50
	9	25.030	24.760	30.637	NT	26.809	0.191	0.71
	10	21.723	21.643	21.650	NT	21.672	0.057	0.26
	β-Actin control
	1	23.140	23.207	23.740	NT	23.362	0.047	0.20
	2	22.983	22.113	22.303	NT	22.467	0.615	2.74
	3	21.880	22.920	23.190	NT	22.663	0.735	3.24
	4	23.130	23.227	23.323	NT	23.227	0.068	0.29
	5	21.023	21.123	21.323	NT	21.157	0.071	0.34
	6	23.710	23.773	24.030	NT	23.838	0.045	0.19
	7	18.923	19.270	19.147	NT	19.113	0.245	1.28
	8	22.403	22.360	22.197	NT	22.320	0.031	0.14
	9	24.040	24.153	27.763	NT	25.319	0.080	0.32
	10	22.290	22.223	22.240	NT	22.251	0.047	0.21

For each sample, the C_T value is the average of results from two to three replicates of the SYBR Green PCR run of that day. NT, not tested.

CV, coefficient of variation.