Fig. 1.

Zebrafish influenza infection and CPC exposure workflow. Zebrafish embryos were collected 15 min post-fertilization and grown at 33 °C. At 48 h post fertilization (hpf), embryos were dechorionated (spontaneously or manually) and injected with IAV. CPC treatment of 0.1 μM in egg water was administered to the embryos at 6, 24, or 48 h post infection (hpi) for a duration of ~60 min before being washed two times to remove remaining traces of CPC from the egg water. Mortality and viral load were assessed every 24 h for five days post-infection (dpi).